截肢手术后疤痕和新的心肌组织评估左心室顶点的P2老鼠显示心肌再生的缺乏。(A)的生存P2的老鼠接受了截肢(AMP;n = 91)(虚假的;n = 51)左心室顶端的一小部分。动物生存的决心立即(0 h), 3小时后(3 h)和手术后的第二天(24小时)。数据意味着±SEM。(B) Stereomicroscopic AMP和虚假的心在21 dpa的照片展示了广泛的存在白斑在外部心室壁手术后21天(黑色箭头)。规模的酒吧,1毫米。(C)心脏的重量P2 AMP (n = 3)和虚假的(n = 3)小鼠决心手术后在不同的时间点。(D)代表)——并在21 dpa trichrome-stained部分P2的心。心脏组织损失是明显的偏差组织纤维(圆)嵌入)的存在强烈的蓝色染色(三色的插图)。虚线标志着病变边界。 Scale bar, 200 μm. Right: Quantification analysis of the scar area in P2 AMP versus SHAM at 21 dpa. ***P < 0.001. (E) Paraffin-embedded resected P2 hearts were sectioned and immunostained for troponin-I and DAPI. Representative images at various time points during the post-AMP process demonstrate an absence of newly formed myocardial tissue. An asterisk indicates the healing area, whereas the dashed line marks the lesion border. Scale bar, 25 μm. (F) Number of proliferating CMs (DAPI+/MF-20+/BrdU+ cells) per 0.1 mm2 counted in paraffin-embedded SHAM and AMP heart sections from BrdU multiple pulse-chase experiments at the indicated dpa. Data are means ± SEM; n = 3. Only fields completely filled with cells were included in the analysis. ns, not significant. Credit:科学的进步(2018)。DOI: 10.1126 / sciadv.aao5553
