全细胞膜片箝记录CA1海马锥体神经元的年龄和年轻的动物展示和突触衰变时间的电流电压关系。(A)被记录的电流电压关系从年轻CA1锥体神经元(11/4细胞/动物)和年龄(9/4细胞/动物)的动物。当细胞被夹在正电压,电流外,更大的电流是观察年轻的动物。附近的潜力是逆转0 mV两个年龄组。当细胞被夹在负电压,电流的减少,符合Mg2 + NMDAR通道的封锁。考试时间和峰值振幅的half-decay NMDAR EPSC在衰老。这些细胞被电压夹在40 + mV。(B)代表八个不同的刺激强度和记录所唤起的痕迹从岁年轻(上)和动物(底部)。(C)降低峰值NMDAR EPSC观察在CA1锥体细胞的刺激强度范围记录年龄动物(实心圆,n = 26/14细胞/动物),相对于细胞年轻动物(开环,n = 20/9细胞/动物)。(D)的平均(±SEM)时间EPSC衰减到50%的峰值三刺激强度最高。 The inset shows the time course of the EPSC, evoked by 40 V stimulation, across all CA1 pyramidal cells recorded from young (gray trace, n = 20/9 cells/animals) and aged (dark trace, n = 26/14 cells/animals) animals. For each cell, the response amplitude evoked by 40 V stimulation was normalized to the peak of the response. Credit: Ashok Kumar, Thomas C. Foster
