S2 for validation of Jedi-1 expression in perineurial glial cells. (B) WT P0 DRG co-stained for Jedi-1 (red), satellite glial marker BFABP (green), and DAPI (blue). (C) WT DRG co-stained for Jedi-1 (red) and PGP9.5 (green) and TOPRO3 (blue). Right shows inset. (D) Right: Primary WT DRG cultures stained for neurons using Tuj1 (green) and Jedi-1 (red). Left: HeLa cells overexpressing mouse Jedi-1 were used as a positive control for Jedi-1 immunocytochemistry in vitro. All images were analyzed in ImageJ version 2.0.0-rc-69/1.52p. Credit: Scientific Reports (2020). DOI: 10.1038/s41598-020-57971-2">
S2 for validation of Jedi-1 expression in perineurial glial cells. (B) WT P0 DRG co-stained for Jedi-1 (red), satellite glial marker BFABP (green), and DAPI (blue). (C) WT DRG co-stained for Jedi-1 (red) and PGP9.5 (green) and TOPRO3 (blue). Right shows inset. (D) Right: Primary WT DRG cultures stained for neurons using Tuj1 (green) and Jedi-1 (red). Left: HeLa cells overexpressing mouse Jedi-1 were used as a positive control for Jedi-1 immunocytochemistry in vitro. All images were analyzed in ImageJ version 2.0.0-rc-69/1.52p. Credit: Scientific Reports (2020). DOI: 10.1038/s41598-020-57971-2" width="800" height="486">
绝地表达于外周胶质细胞和内皮细胞。（一个)成人(8-12周大)WT坐骨神经切片，Jedi-1染色(品红色)，DAPI染色(蓝色)。插图1和2显示了血管中的绝地表情。看到补充图。S2验证Jedi-1在神经周围胶质细胞中的表达。（B) WT P0 DRG共染Jedi-1(红色)、卫星胶质标记BFABP(绿色)和DAPI(蓝色)。（C) WT DRG共染Jedi-1(红色)、PGP9.5(绿色)和TOPRO3(蓝色)。正确的显示了插图。（D右图:用Tuj1(绿色)和Jedi-1(红色)染色的原代WT DRG培养神经元。左图:过表达小鼠Jedi-1的HeLa细胞作为Jedi-1免疫细胞化学阳性对照在体外．所有图像在ImageJ 2.0.0-rc-69/1.52 2p版本中分析。信贷:科学报告(2020)。DOI: 10.1038 / s41598 - 020 - 57971 - 2
S2 for validation of Jedi-1 expression in perineurial glial cells. (B) WT P0 DRG co-stained for Jedi-1 (red), satellite glial marker BFABP (green), and DAPI (blue). (C) WT DRG co-stained for Jedi-1 (red) and PGP9.5 (green) and TOPRO3 (blue). Right shows inset. (D) Right: Primary WT DRG cultures stained for neurons using Tuj1 (green) and Jedi-1 (red). Left: HeLa cells overexpressing mouse Jedi-1 were used as a positive control for Jedi-1 immunocytochemistry in vitro. All images were analyzed in ImageJ version 2.0.0-rc-69/1.52p. Credit: Scientific Reports (2020). DOI: 10.1038/s41598-020-57971-2" width="800" height="486">
