图1所示。Tnc在野生型视网膜缺血的早期诱导。(A-D)对照组和缺血WT组3和7天的视网膜横切面Tnc染色图像。免疫组化显示Tnc信号在IPL和OPL以及NFL(红色)。细胞核用TO-PRO-3反染(蓝色)。(E) Tnc+染色面积在第3天显著增加，提示缺血损伤后早期诱导Tnc。然而，在第7天，Tnc染色明显减少。WT CO组设置为100%。(F-I)在I/R后3天，相对蛋白定量显示较大的Tnc亚型显著上调。(J-M) 7天后观察到Tnc蛋白水平。 (N) No differences of the Tnc mRNA expression were noted at both points in time after ischemia. Data were analyzed via Student t-test and presented as mean ± standard error mean (SEM) in panels (E,G–I,K–M). For RT-qPCR, groups were compared using the pairwise fixed reallocation and randomization test in panel (N). These data are shown as median ± quartile ± minimum/maximum. *p < 0.05; **p < 0.01. n = 4–5/group. Scale bar = 20 μm. ONL: outer nuclear layer, OPL: outer plexiform layer, INL: inner nuclear layer, IPL: inner plexiform layer, GCL: ganglion cell layer, NFL: nerve fiber layer. Credit:神经科学前沿(2021)。DOI: 10.3389 / fnins.2021.642176
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