图1:SARS-CoV-2感染与大脑中弦血管增加有关。a-c，在sars - cov -2感染患者的大脑中，空的基底膜管，也称为弦血管(箭头)，在额叶皮层增加。染色基底膜标记物IV胶原(coll IV)和内皮标记物CD34。a和b中的代表性图像来自c中的数据集。a，比例尺，50 μm。b，用正交视图放大血管的z-堆栈的最大投影，以排除这些是毛细血管的部分切片。比例尺，3 μm。c，每个图像体积的字符串血管量化。对照组23例，新冠肺炎患者17例。d，免疫染色显示sars - cov -2感染患者皮质切片中caspase-3阳性活性血管数量(N = 6)高于对照组(N = 6)。给出了代表性图像和量化结果。 Scale bar, 20 µm. e,f, SARS-CoV-2-infected hamsters developed an increased number of string vessels as shown by co-staining for coll IV and the endothelial marker caveolin-1. e, Representative images of coll IV and caveolin-1 in the cortex of hamsters 4 d post infection (p.i.) with SARS-CoV-2 and of uninfected hamsters. Scale bar, 50 µm. f, Quantification of string vessel lengths as a percentage of total vessel length in SARS-CoV-2-infected hamsters at 4, 7 and 24 d p.i. and in uninfected controls (N = 4 hamsters per group). g,h, SARS-CoV-2-infected K18-hACE2 mice developed an increase in string vessels as shown by co-staining for coll IV and caveolin-1. g, Quantification of string vessel lengths as a percentage of total vessel length in SARS-CoV-2-infected K18-hACE2 mice 2 d p.i. (N = 3 mice) and 7 d p.i. (N = 3 mice) and in uninfected controls (N = 5 mice). h, Representative images of coll IV and caveolin-1 in the cortex of K18-hACE2 mice 7 d p.i. and of uninfected K18-hACE2 animals. Scale bar, 50 µm. *P < 0.05, **P < 0.01. Means ± s.e.m. are shown. N denotes the number of patients or animals. Credit: DOI: 10.1038/s41593-021-00926-1
