高唾液酸结合免疫球蛋白样凝集素(Siglec)-15在急性髓系白血病(AML)患者循环血细胞表面的表达。急性髓系白血病患者外周血白细胞制剂中Siglec-15的细胞表面表达与健康外周血白细胞相比，12例AML患者中有9例显示出明显的Siglec-15细胞表面表达。(A)来自“患者A”的代表性图显示AML母细胞细胞表面sigec -15的高水平表达[占所有外周血单个核细胞(pbmc)的36%]。Siglec-15与CD14和人白细胞抗原- dr同型(HLA-DR)共表达。阴性对照:小鼠免疫球蛋白G1 (IgG1)同型抗体。(B)来自“患者B”的代表性图(左图)显示AML细胞表面CD33染色呈阴性，尽管20%的细胞Siglec-15呈阳性。比较“病人B”AML母细胞表面CD33和Siglec-15表达的直方图(右图)(同型对照染色，填充直方图;抗cd33 /Siglec-15抗体染色，开放直方图)。(C)来自“患者C”的代表性图显示，10%的AML母细胞在细胞表面表达Siglec-15，其中大部分是CD33+CD14+HLA-DR−。 (D) Percentage of total circulating PBMCs expressing cell-surface Siglec-15 from 14 patients with AML (open circles) and nine healthy donors (solid squares). Normalised percentage positive expression shown. Mann–Whitney two-tailed t-test, median and interquartile ranges are indicated (***P = 0·0002). (E) Correlation between normalised percentage Siglec-15+ and CD33+ populations from 10 patients with AML. Pearson’s correlation: r = 0·9354, P < 0·0001, two tailed test. (F) Geometric mean of fluorescence intensity (gMFI) values of A9E8 binding normalised against isotype binding for PBMCs expressing cell-surface Siglec-15 from 12 patients with AML (open circles) and 12 healthy donors (solid squares). Mann–Whitney two-tailed t-test, median and interquartile ranges are indicated (***P = 0·0002). Credit: DOI: 10.1111/bjh.17496
