图1:死后ASD患者PFC中ASH1L的表达和H3K4me3水平显著降低，这在年轻小鼠PFC中通过敲除ASH1L重复。a定量PCR数据显示对照组与特发性ASD患者死后PFC组织中ASH1L mRNA水平。n = 12人(10 M, 2 F) /组* * p < 0.01, t双尾检验。b Western blot数据显示对照组与ASD患者死后PFC中H3K4me3和H3K36me2水平。n = 12人(10 M,2 F)/组，*p < 0.05，双尾t检验。c定量PCR数据显示转染Ash1L shRNA或打乱(sc) shRNA的N2A细胞中Ash1L mRNA水平。n = 6/组。***p < 0.001，双尾t检验。d小鼠立体定向注射Ash1L shRNA AAV (gfp标记)的共聚焦图像，显示病毒感染的PFC区(DAPI，蓝色)。比例尺:300 μm。 e, f Quantitative PCR and Western blot data showing Ash1L mRNA and protein levels in PFC of mice (5-week-old) with the stereotaxic injection of Ash1L shRNA vs. sc shRNA AAV. Ash1L mRNA, n = 15 mice(8 M,7 F) for sc shRNA group, n = 14 mice(7 M,7 F) for Ash1L shRNA group; Ash1L protein, n = 8 mice(4 M,4 F)/group, ***p < 0.001, two-tailed t test. g Western blot data showing H3K4me3, H3K36me2, and H3K36me3 levels in PFC infected with Ash1L shRNA or scrambled shRNA AAV. n = 8 mice (4 M,4 F)/group, ***p < 0.001, two-tailed t test. h Representative confocal images and quantification of immunostaining of H3K4me3 (red) in PFC neurons infected with Ash1L shRNA or a scrambled shRNA AAV (GFP+ , green). n = 20 images/4 mice (2 M,2 F)/group, ***p < 0.001, two-tailed t test. Scale bar: 10 μm. i, j Representative confocal images and quantification of immunostaining of NeuN (red) and DAPI (blue) in PFC neurons infected with Ash1L shRNA or a scrambled shRNA AAV (GFP+ , green). Slices were collected at 8–9 days postinfection. n = 20 images/4 mice (2 M,2 F)/group. Scale bar: 20 μm. All the full Western blots are included in Supplementary Fig. 7a–c. Data are presented as mean values ± SEM. Detailed statistical data are provided in a Source Data file. Credit: DOI: 10.1038/s41467-021-26972-8
