图1:转移性乳腺癌细胞选择性BM血管利基。实验工作流程:4 t1.2-rfp细胞orthotopically植入BALB / c小鼠。主2周后,切除肿瘤骨收集在伦理端点,低温贮藏然后immunolabelled。b:瓷砖扫描覆盖整个股切片的Z-depth ~ 100µm。骨头是DAPI染色(灰色),4 t1.2传播肿瘤细胞(转轨;洋红色),Endomucin (EMCN;黄色)和CD31(青色)。底部:四个例子(对应于(i)−(iv)从上图)4 t1.2转轨国家作为单独或集群细胞(n = 11老鼠)。规模的酒吧:1毫米(上),50µm(底部)。c 3 d图像覆盖整个股BM应用4 t1.2转轨国家(红色)、CD31(绿色)和Col1a1(灰色)小鼠(n = 11)。 Scale bar, 1 mm. d Distance to the nearest blood vessel or bone surface for individual 4T1.2 DTC or simulated random spot. A total of 1426 DTCs were analyzed from 11 different mice. P values, two-tailed unpaired t tests. e, f Percentages of 4T1.2 DTCs and random spots within 10 µm of a bone surface (e) or blood vessel (f). For (e) and (f), a total of 655 DTCs and 758 DTCs were analyzed in 8 and 6 mice, respectively. P values, two-tailed unpaired t tests. g Optical section and surface rendering from 3D image of a 4T1.2 micrometastasis (magenta) in bone marrow immunostained for EMCN (yellow). In surface rendering, 4T1.2 cells and blood vessels were pseudo-colored to yellow and gray, respectively (n = 11 mice). BV blood vessel. Scale bars: 50 µm (left), 10 µm (middle and right). All data reflect mean ± s.e.m. Source data are provided as a Source Data file. Credit: DOI: 10.1038/s41467-021-26556-6
