图1:特征的抗原CD8 + T细胞在急性SARS-CoV-2感染和记忆阶段。一、研究设计的概述。PBMC,外周血单核细胞。b,代表块CoV2-Dex染色。PE、藻红蛋白。父情节显示比例的人口数量。c,频率CoV2-Dex +细胞健康的捐赠者和COVID-19患者在急性感染,感染后6个月和1年。每个点代表一个独立的捐赠者的表示的计算(n = 10健康,n = 37急性,n = 32 6个月,n = 29在感染后1年)。P值显示。d,频率的线性回归CoV2-Dex +细胞在感染后6个月作为CoV2-Dex +细胞频率的函数在急性感染(n = 11)。 The P value was calculated with t-statistic. e, Uniform manifold approximation and projection (UMAP) plots of marker expression for up to 2,000 CD8+ T cells from each sample collected during acute infection (n = 37) analyzed by spectral flow cytometry. Regions with high marker expression appear in red. An overlay of CoV2-Dex+ cells (red) and total CD8+ T cells (gray) is shown in the top left. f, Representative histograms showing expression of selected markers on CoV2-Dex– and CoV2-Dex+ cells. g, Frequency of Ki-67+, HLA-DR+, granzyme B+, CX3CR1+ and CD127+ cells in CoV2-Dex– (gray) and CoV2-Dex+ cells during acute infection and 6 months and 1 year after infection. Analysis was conducted on paired samples from acute infection versus 6 months and/or 1 year after infection (n = 28 acute, n = 24 6 months, n = 29 1 year). The gray lines connect individual donors sampled at different timepoints. P values were calculated using a Wilcoxon–Mann–Whitney test in c and g and corrected for multiple comparisons in g. All tests were performed two-sided. Credit: DOI: 10.1038/s41586-021-04280-x
