图1:不一致的蛋白质组学和转录组学特征揭示了SC从静止到激活转变过程中潜在的翻译控制机制。facs分离SCs的蛋白质组学分析工作流程示意图。SCs来自Pax7-nGFP小鼠。从0.5% pfa灌注的小鼠中筛选出QSCs。从未灌注的小鼠中筛选出fiss。从1.2% BaCl2损伤3天的小鼠中筛选出损伤ASCs (iASCs)。培养ASCs (cASCs) 2天。(n = 2个独立实验)。b QSCs、fcs、iASCs和cASCs蛋白表达的层次聚类(以谱数为单位)。c QSCs、fscs、iASCs和cASCs细胞周期、静止和激活特异性蛋白的代表性表达热图。 d Heatmap of representative expression for fatty acid oxidation and glycolysis-related proteins of QSCs, fiSCs, iASCs, and cASCs. e Heatmap of representative expression for transmembrane protein/receptors of QSCs, fiSCs, iASCs, and cASCs. f, g Analysis of transcriptome and proteome on QSCs and cASCs (proteome comparison) and iASCs (transcriptome comparison). f Scatter plot of the fold change of RNA and protein comparing QSCs to ASCs. Heatmap shows the RNA and protein expression of representative genes in the lower right quadrant of the scatter plot. g Functional enrichment analysis of genes from the lower right quadrant of f by g:Profiler. The g:Profiler uses a hypergeometric test to measure the significance of functional terms in the input gene list. Credit: DOI: 10.1038/s41467-022-28612-1
