波形蛋白在肿瘤内皮细胞中过表达，细胞外存在。A，B波形蛋白mRNA（a; n = 3; qPCR）和蛋白质（B; n = 4;流式细胞仪）在人类结肠肿瘤（TEC）和正常结肠（NEC）中分离的内皮细胞（EC）中的表达。C中的c波形蛋白mRNA表达在人（结肠，n = 13;神经胶质瘤，n = 2）和鼠（神经胶质瘤，n = 7;黑色素瘤，n = 2）肿瘤中分离的EC中。D蛋白质组学分析分泌组中的人类正常结肠和结直肠癌组织（左图； n = 21（正常），n = 4（I级），n = 8（II级），n = 5iv））和总细胞内波形蛋白（右图； n = 15（正常），n = 15（CRC I – IV））。数据表示为A – D中的平均值±SEM。p值代表配对的t检验（A，C，D右图），未配对的t检验（B）和单向ANOVA（D左图）。固定和通透的HUVEC（左图）和活的完整HUVEC（右图）的E免疫荧光染色。插图：负面对照。显示了至少三个独立实验的代表性图像。 f Schematic representation of vimentin localization (in green). g Western blotting of total cell lysate, ECM deposit, and secretome of HUVEC. Representative sections of at least three independent experiments are shown. h Global proteomics analysis (n = 1) of HUVEC lysate, secretome, and ECM deposit. i (Left) Proportion of known tumor EC markers (TEC, red) among externalized proteins. (Right) Secretion mechanisms among externalized proteins. j Protein–protein interaction analysis using STRING of externalized TEC markers. Opacity levels of the nodes are proportional to secretion abundance. k Effect of angiogenesis inhibitors and cytokines on vimentin secretion. Relative secretion is color-coded according to the legend right of the panel, and agent types are color-coded according to the legend below the panel. l Schematic of different cellular protein secretion pathways. m Effect of different protein secretion mediators on vimentin secretion. Legend as in k. Data are color-coded as mean values of relative secretion in k and m; numbers of samples are presented in the Source Data file. *p < 0.05 based on Kruskal–Wallis test with Dunn’s multiple comparison test correction for k and m. Source data are provided as a Source Data file. Credit:自然通讯（2022）。doi：10.1038/s41467-022-30063-7
