SOX1阳性背侧前脑神经上皮细胞的诱导与维持。TGFbR和BMPR抑制剂存在下hPSCs的神经诱导方案。经皮质神经诱导后，NES细胞在SOX2阳性细胞中表达NESTIN b, TUBB3阴性细胞中表达SOX1 c, FOXG1 d, SOX2阳性细胞中表达PAX6 e。用5个生物独立细胞系重复实验。Scalebar:经不同因素组合(n = 3个独立细胞株，每组每个细胞株10个数点，红柱为均数±SEM，单向方差分析，Tukey’s检验)处理的hES (H1, H9, CA1)源性cNESCs传代5培养中SOX1阳性细胞比率为25 μm f。g cNESCs细胞数量变化的归一化模型(数据以H1-、H9-、ca1来源细胞的均值±SEM表示，n = 3个独立细胞系，2向方差分析，Dunnett’s检验)超过10代(30天)。h H1衍生的cenccs (p36)在培养第4天(上)和第14天(下)在4F形成莲座结构的相位对比图像。用4个生物独立细胞系重复实验。比例尺:50 μm。i:在播种前以200个细胞/平方厘米密度在4F中培养的cNESCs的集落形成试验。 j Schematic presentation of developmental signaling pathway components targeted in our assay, protein ligands are in bold and chemical inhibitors are in italic, 4F components are in red. k Quantification of cell number changes after 96-h treatment of cNESCs with indicated ligands or chemical inhibitors compared to 4F condition (n = 3 independent experiments, data are presented as mean ± SEM, 1 way ANOVA, Tukey’s test, white bars are p < 0.01, grey bars are not significantly different from control, red line indicates starting cell number). Credit:自然通讯(2022)。DOI: 10.1038 / s41467 - 022 - 29839 - 8
多伦多大学(University of Toronto)和西奈健康中心(Sinai Health)的科学家表示，他们发现了一种控制神经干细胞命运的新方法，使研究人员离解开受伤或中风后如何修复大脑的谜团又近了一步。
