Fbxl-1 (ok3741)虫神经运动功能受损。(A)咽泵率实验概述(补充视频1).斜照度解剖显微镜下观察WT秀丽隐杆线虫动物;磨床在终端灯泡上显示为一个白点(箭头)。(B) fbxl-1(ok3741)和WT虫在1、6天成虫期咽部泵血率(1天成虫期每株n = 20;6天成虫期每株N = 25)。Bonferroni的修正方法被用来解释多重比较，重要的发现仍然成立。(C) fbxl-1(ok3741)和WT在琼脂板上的体弯曲率(补充视频2和3). n =每个菌株25只;进行5个生物重复。(D-F)蠕虫在液体介质中的游动活动分析。(D和E)将5只蠕虫转移到玻璃载玻片上的每一滴液体介质(比例尺:1毫米)中。 Activity of all 5 worms in aggregate was recorded, and pixel change was analyzed using ZebraLab. (F) fbxl-1(ok3741) worm swimming activity was significantly lower than WT worms (Supplemental Videos 4 and 5). Each dot indicates the total activity of 5 worms in a single technical replicate. n = 12 for WT; n = 11 for fbxl-1(ok3741); with a total of 60 WT and 55 fbxl-1(ok3741) worms analyzed across 4 biological replicates. (G–J) Thrashing activity assay. (G) Each fbxl-1(ok3741) and WT worm was transferred to 5 μL liquid media for individual worm activity analysis by wrMTrck plugin (ImageJ). The graph shows the BBPS, which were significantly decreased in fbxl-1(ok3741) worms as compared with WT worms. Each dot indicates activity of an individual worm. n = 36 each strain. (H and I) Representative traces of worm movements detected by wrMtrck analysis. fbxl-1(ok3741) worms showed less activity and uncoordinated movements than WT worms. (J) Higher-magnification images of representative individual fbxl-1(ok3741) and WT worm traces. Data are shown as the mean ± SD. Significance was determined using unpaired Student's t test. *P < 0.05, **P < 0.01, ***P < 0.001. Credit:江森自控的洞察力(2022)。DOI: 10.1172 / jci.insight.156346
