PEMF通过调节成骨细胞改善局灶性辐射诱导的骨丢失。(A)聚焦辐射和PEMF注入示意图。(B)大鼠股骨远端(n = 8根)代表性显微计算机断层扫描(micro-CT)图像和定量分析。(C) H&E染色显示大鼠胫骨近端小梁区(每组n = 8个胫骨)。(D)基于钙黄素/茜素红标记的动态骨组织形态分析(每组n = 8股)。(E) Runx2免疫组化染色(n =每组8个胫骨)。(F) TUNEL染色检测骨表面成骨细胞凋亡(每组n = 8个胫骨)。(G) H&E染色显示空骨细胞陷窝(每组n = 8个胫骨)。(H) TUNEL染色检测凋亡骨细胞(每组n = 8个胫骨)。(I、J)有或无PEMF刺激局灶照射大鼠骨细胞数量和骨细胞树突数量的扫描电镜观察(n = 8股/组)。 (K) TRAP staining for labeling osteoclasts on bone surface (n = 8 tibiae per group). (L and M) Serum ELISA assays for bone formation markers (OCN and P1NP) and bone resorption markers (CTX-1 and TRACP-5b) (n = 8 rats per group). Graphs represent means ± SD. (B to K) *P < 0.05; **P < 0.01. Statistical analyses were done using two-way ANOVA with Bonferroni’s posttest. (L and M) **P < 0.01. Statistical analyses were done using Student’s t test. Scale bars, 200 μm (C and K), 100 μm (I), 50 μm (E to H), 20 μm (D), and 5 μm (J). Credit:科学的进步(2022)。DOI: 10.1126 / sciadv.abq0222