双重NDR激酶在神经元的损失会导致神经退化。(一)线图显示NDR1 KO的平均体重,NDR2 KO, NDR1/2 KO和控制小鼠6周。重量的差异基因型组之间在每个时间点用普通单向方差分析或克鲁斯卡尔-沃利斯测试进行了分析。n = 20 - 30老鼠/组。(B)图说明NDR1 KO的生存概率,NDR2 KO, NDR1/2 KO和控制小鼠6 mo的年龄。n = 20 - 30老鼠/组。(C, D)免疫荧光染色的脑片NDR1/2 Ctip2 KO和控制老鼠P20或12周的年龄。白中带箭头的线DAPI图像显示皮层的厚度,和白色的带箭头的线在Ctip2图像显示的上层I-IV皮层的厚度。酒吧规模:200μm。图表显示皮层厚度的量化,并使用配对t测试数据进行了分析。 n = 18 measurements from three mice/genotype. (E) Immunofluorescence staining of GFAP in brain slices of 12-wk-old NDR1/2 KO and control mice. White arrows show areas with increased GFAP signal in NDR1/2 KO mice. (F) Western blot analyses of GFAP levels in lysates from the cortex of 6-wk-old mice. GAPDH was used as a loading control. The graphs show quantifications of the GFAP bands normalized against the GAPDH levels, and the data were analyzed using an ordinary one-way ANOVA with Tukey’s post hoc test. n = 3–5 mice/group. (G) Immunofluorescence staining of GFAP and the microglial marker Iba1 in the CA1 area of the hippocampus. White arrows indicate cells expressing the above-mentioned markers. Scale bars: 50 µm. (H) Images from brain slices of 12-wk-old Thy1-YFP–expressing mice in the CA1 area of the hippocampus. White arrowed lines indicate the stratum radiatum, where CA1 neuron dendrites are visible in YFP. The graph shows quantification of the hippocampal thickness (marked by the dashed yellow line—including stratum oriens, the CA1 cell body area, stratum radiatum and stratum lacunosum-moleculare), and the data were analyzed using a paired t test. n = 18 measurements from three mice/genotype. (I) Images from brain slices of Thy1-YFP–expressing mice in the CA1 cell body layer. The white arrow shows membrane protrusions present in NDR1/2 knockout neurons. Credit:生命科学联盟(2022)。DOI: 10.26508 / lsa.202201712
