通过EP2受体阻断PGE2信号通路可以增加老年小鼠的AM数量。a-c龄(18-22个月)C57BL/6小鼠每天注射7次EP2拮抗剂。同时，BrdU (0.8 mg/mL)在饮用水中自由给予。然后从BALF中收集AMs (CD45+ D11c+ SiglecF+)，流式细胞术分析BrdU的加入(提示增殖)，Annexin V染色(提示凋亡)。a灌洗的AMs细胞总数。N = 6/组。b BrdU+的AMs百分比。N = 5/组。c膜联蛋白V+的AMs百分比。N = 6/组。 d–f Aged mice were given clodronate-loaded liposomes to deplete resident AMs prior to an adoptive transfer of CFSE+ WT or EP2 KO AMs as depicted in (d). e, f AMs were analyzed by flow cytometry 4 weeks following transfer for CFSE dilution, a marker of proliferation n = 6/group. f Representative histogram of CFSE MFI. For panels a–e, statistical significance analyzed by Mann–Whitney, error bars represent SEM and each point represents a biological replicate. Credit:自然通讯(2022)。DOI: 10.1038 / s41467 - 022 - 34593 - y
