Spns2缺失影响淋巴细胞封闭和淋巴结的血管结构。T细胞区(cd3 -绿色)和B细胞区(b220 -洋红色)在WT、Spns2- / -和Spns2- mx1cre小鼠宫颈LNs中具有代表性的共聚焦图像。每个基因型N = 3。B .与WT相比，Spns2- / -和Spns2- mx1cre小鼠的LNs中T细胞和B细胞的限制是无序的，在敲除小鼠的B细胞区发现更多的T细胞。每个符号代表三种基因型小鼠的一幅图像。显示的数字是P值。采用单因素方差分析。C用MECA-79对WT、Spns2−/−、Spns2f/f(不含Mx1Cre)和Spns2-Mx1Cre小鼠宫颈LNs进行免疫染色。箭头为WT和Spns2f/f小鼠hev的内膜。每个基因型N = 3。 D Quantification of Meca-79 density in cervical LNs from WT, Spns2−/−, Spns2f/f (without Mx1Cre), and Spns2-Mx1Cre mice with MECA-79. ns not significant; t test. E Meca-79 localization was dysregulated in HEVs of Spns2−/− and Spns2-Mx1Cre mice. Meca-79 (red) and CD31 (green) antibody. F LYVE-1 staining of the lymphatic vessels in inguinal LN of indicated genotypes. G Quantification of density of lymphatic vessels in the subcapsular regions of LNs of Spns2−/− and Spns2-Mx1Cre mice and control mice. Each symbol represents one image from three mice for each genotype. ns not significant; t test. Credit:细胞和分子生命科学(2022)。DOI: 10.1007 / s00018 - 022 - 04573 - y
