Pld3缺失导致轴突球状体减少,改善神经回路功能。a,被编码Pld3或对照sgrna的AAV病毒感染后,基底前脑中向皮层投射的胆碱能神经元示意图(左)。右,双光子图像显示从基底前脑(红色)投射出的基底前脑轴突(红色)与gcamp6f标记的皮层神经元(绿色)混合在一起。比例尺,100 μm。b, gcamp6f标记皮层神经元的代表性双光子图像(紫色点)。比例尺,50 μm。c,单个皮质神经元的原始钙痕迹示例。d, 30分钟成像过程中单个神经元的单细胞峰值计数。每个点代表同一只老鼠中所有细胞的平均刺突数。小提琴图显示了所有单个神经元的spike计数分布。 e, Pairwise mutual information grouped by the distances between neurons. Data are mean ± s.e.m. Two-way analysis of variance was used to compare between groups. f, Neuron cluster size distributions classified by activity patterns (Louvain clustering; Methods). g, Quantification of population entropy (a measurement of temporal variance of the firing pattern) from each mouse imaged. For d,e and g, n = 4, n = 4 and n = 6 mice in the WT group, 5xFAD with control sgRNA group and 5xFAD with Pld3 sgRNA group, respectively. For f, n = 67, n = 21 and n = 45 clusters in the WT group, 5xFAD with control sgRNA group and 5xFAD with Pld3 sgRNA group, respectively. For d,f and g, statistical analysis was performed using one-way analysis of variance to compare among groups and the P values indicate a post hoc comparison between the groups, with Sidak’s correction for multiple comparisons. For d and g, the bars indicate the group mean. Credit:自然(2022)。DOI: 10.1038 / s41586 - 022 - 05491 - 6