斑马鱼中slf2和smc5的缺失会导致小头畸形和异常的颅面模式。a顶部:受精后3天获得的代表性侧面亮场图像(dpf);白色虚线形状表示头部尺寸测量。下图:−1.4col1a1前区GFP信号的代表性腹侧图像:3 dpf时的egfp转基因报告幼虫。白色虚线显示颈玻璃角。b侧封头尺寸测量的量化。将两种独立的针对slf2的sgrna与Cas9一起或不一起注射幼虫;N = 3个独立实验(从左到右;56、37、37、36、36个幼虫/批)。c颈玻璃角的定量。 Larvae were injected with two independent slf2 sgRNAs: n = 3 independent experiments (left to right; 39, 42, 30, 20, 44 larvae/batch). d Top: Representative lateral bright field images at 3 dpf. Bottom: Representative ventral images of GFP signal in the anterior region of −1.4col1a1:egfp smc5 sgRNA1 transgenic larvae at 3 dpf. e Quantification of lateral head size measurements in 3 dpf larvae (as shown in panel a); n = 3 independent experiments (left to right; 50, 50, 52, 46, 53, 38 larvae/batch). The chart shows two independent experiments for sgRNA1 and sgRNA2 with a vertical line grouping independent controls with test conditions. f Quantification of the ceratohyal angle. Larvae were injected with two independent smc5 sgRNAs: n = 3 independent experiments (left to right; 34, 53, 37, 62, 28, 48 larvae/batch). The chart shows two independent experiments for sgRNA1 and sgRNA2 with a vertical line grouping independent controls with test conditions. g Left: Representative lateral bright field images of WT control and slf2−−/突变体在3 dpf。右图:3 dpf WT对照和slf2中侧头尺寸测量的量化−−/突变幼虫(见a);N = 3个独立实验(从左到右;10、12、12只幼虫/批)。(a, b):(左上)白色虚线形状表示测量的头部尺寸;(左下)白色虚线表示所测角。MK Meckel软骨，CH角鳃软骨(分别用箭头表示)，CB角鳃弓(星号)。比例尺为300 μm，面板间尺寸相等。误差条表示平均值的标准偏差。统计学差异由未配对的学生t检验(双侧)确定。信贷:自然通讯(2022)。DOI: 10.1038 / s41467 - 022 - 34349 - 8
