结扎后小鼠下颚骨变性，结扎后下颚骨再生。(Aab)结扎牙周炎模型。用杜蒙钳将5-0丝线穿过上颌第一磨牙、第二磨牙、第三磨牙的牙间隙。缝线用三结扎牢，多余的缝线用弹簧剪刀剪断，如图(a)和显微照片(b)所示。摘自参考文献(31)。(Ba-c)结扎期为10天，第0天、第5天和第10天的小鼠颌部显微ct扫描。对照组图像代表不接受药物治疗的组(n = 3)(对照组，上行)，实验组图像代表接受药物治疗的组(n = 3)(实验，下行)。红色箭头表示上颌左第二磨牙，骨性退变延伸至邻近的第一和第三磨牙。为了视觉清晰，图像颠倒180度(现在:下颌骨顶部，上颌骨底部)。(C)在结扎后的第5天和第10天，对所有实验动物的变性区域进行了测定。y轴为:骨丢失面积(mm2); (n = 7); error bars represent standard errors; for days 0–5, p = 0.00289; for days 0–10, p = 7.33084 × 10−11; and for days 5–10, p = 3.60818 × 10−7. (**) Represents p less than 0.01. In (D), a graph of area of bone growth is seen for mice post–ligature but not given drug (no drug control) vs. mice given drug (DPCA-PEG). Mice were injected with DPCA-PEG subcutaneously on days 0 and 8 after ligature placement and removal. MicroCT scans were obtained on day 15 of the experiment (day 5 post-ligature) and on day 30 (day 20 post-ligature). Here, a statistical analysis of the area of bone growth (mm2) is seen. Significant differences are found between the no drug control mice (n = 10) and DPCA-PEG-treated mice (p = 0.00253) on day 15 (blue bars) (n = 12). The same is true on day 30, where DPCA-PEG-treated mice showed highly significant differences from non-drug-treated controls (p = 0.00612) (red bars). Area analysis was performed as described in the Materials and Methods. The Y-axis = Area of bone regrowth (mm2); the error bars represent standard errors; and p values are represented as (*) = p less than 0.05; (**) = p less than 0.01. Mouse jaws analyzed were n = 10 for ligature, no drug; n = 12 for ligature/plus drug. In (E) micro-CT data shows a representative mouse maxilla which had ligature removed at day 10, scanned on day 10 and then re-scanned on day 30 (Ea,b) as compared to a representative mouse maxilla receiving ligature and DPCA-PEG drug seen on day 10 and day 30 (Ec,d). The level of regrowth in the drug-treated group shows an almost, if not complete, return to what is seen before the start of the experiment (da0) (Ed,e). In (F), there was no change in bone histology approximately 6 months later. Mice injected with DPCA-PEG drug were kept for additional observation as they aged. Over six months after the da 30 scan, mice were rescanned (upper panel) and then compared to the day 30 scan by overlaying the two scans on da 30 and da 220 (lower panel). The black line is the da 30 scan and the red line is the da 220 scan. Though shown as two lines, they are exactly overlapping. This result is representative of three mice. Credit:牙科医学前沿(2022)。DOI: 10.3389 / fdmed.2022.992722
的临床前研究重点研究了牙周病的一个模型，它引起牙龈和骨质流失这会导致牙齿脱落。在老年人中，这种疾病不仅会引起疼痛和不适，而且是牙齿脱落的最常见原因，影响30-60%的成年人。但是，实验研究结果表明，时间的释放药物这种被称为1,4- dpca的牙齿完全修复了病变的牙龈和周围的颌骨，完全防止了牙齿脱落。研究结果由LIMR的Heber-Katz团队(Main Line Health的一部分)于11月发表在该杂志上牙科医学前沿．
