a，四个MB亚组(G3, 4组(G4)， SHH和WNT)和未受影响(正常)组织中基因表达的热图。两方面的变化;错误发现率(FDR) < 0.05。b，显示g3相关基因与表观遗传基因之间SMARCD3重叠表达的维恩图。c，使用MB患者的转录组数据显示SMARCD3 mRNA表达的小提琴图。方差分析。d，均匀流形近似和投影(UMAP)可视化(左)和小提琴图(右)显示基于25例mb患者scRNA-seq数据的SMARCD3 mRNA表达。e，箱线图显示SMARCD3表达水平(nG3 = 14, nG4 = 13, nSHH = 15, nWNT = 3)。f，基于SMARCD3 mRNA表达水平比较所有MB亚组(左)和仅G3(右)患者的Kaplan-Meier生存曲线。g，左，MB组织微阵列中SMARCD3水平IHC染色的代表性图像。对，基于SMARCD3水平比较所有MB亚组和G3患者生存分数的log-rank检验。h, GO分析smarcd3相关基因在MB中的十大生物学通路。 i, Density plots (top) and boxplots (bottom) showing the association between metastasis status (0, no metastasis; 1+, metastasis at diagnosis) and SMARCD3 mRNA (n0 = 397, n1+ = 176) and protein (n0 = 23, n1+ = 20) expression levels in primary MB samples. j, RT–qPCR (top) and immunoblotting (bottom) analyses showing SMARCD3 mRNA (n = 3) and protein levels in six G3 MB cell lines. k, Representative haematoxylin and eosin (H&E) images showing primary tumors (yellow dashed lines) and brain and spinal metastatic tumors (red dashed lines) in six orthotopic xenograft models derived from G3 MB cell lines. Images are representative of three independent mice, with similar results obtained (k). Each dot represents one bulk sample (c,e,i) or one cell (d). n represents the number of human patients (a,c,e,f,g,i) or biologically independent samples (j). Data are presented as the mean ± s.d. P values were calculated using two-tailed Welch’s t-test with FDR correction (c,e,i) or two-tailed accumulative hypergeometric distribution (h). Credit:自然细胞生物学(2023)。DOI: 10.1038 / s41556 - 023 - 01093 - 0
