BVES的破坏损害了小鼠的体重增加和肌肉功能。所有动物实验均在具有C57BL/6N基因背景的WT和BVES-KO雄性小鼠中进行。a用抗BVES和DAPI抗体染色WT和BVES- ko小鼠(4月龄)GA肌肉的免疫荧光图像。比例尺:100µm。(每个基因型n = 4)。b 4月龄WT和BVES-KO雄窝伙伴的代表图像。c，雄性BVES-KO小鼠和年龄/性别匹配的WT小鼠2 - 5月龄体重增加情况。双尾配对学生t检验。d WT和BVES-KO雄性小鼠的Kaplan-Meier生存曲线。e自愿轮跑BVES-KO和年龄匹配的WT雄性小鼠(4个月大)。 f, g Endurance capacity test performed by treadmill running showing running distance (f) and time to exhaustion (g) in BVES-KO (n = 5) and WT (n = 5) male mice (4 months of age). Two-tailed unpaired Student’s t test. h The number of dropouts to test the capacity of recovery from muscle injury on the treadmill in BVES-KO and WT male mice (6 months of age). Two-tailed paired Student’s t test. i Tetanic torque measurements of the posterior compartment muscles of BVES-KO and WT male mice in age-dependent manner (2-month age: WT (n = 11), BVES-KO (n = 9); 4-month age: WT (n = 9), BVES-KO (n = 9); 6-month age: WT (n = 9), BVES-KO (n = 8)). ns indicates no significant difference. Two-way ANONA with Tukey’s multiple comparisons test. Data are mean ± SEM. Source data are provided as a Source Data file. Credit:自然通讯(2023)。DOI: 10.1038 / s41467 - 023 - 37496 - 8
