SIX2 hFK + CITED1 +细胞和WT有不同的转录签名。A、B)高碘酸希夫(不是,左,整幅图像)和圆)(对,特写图片)染色hFK(10,编剧协会)和WT # 4 (B,有利的III期)显示肾发生的区域(白色虚线)和微分结构(第二个面板:输尿管的花蕾,乌兰巴托;帽间质厘米;小管、肾小球和hFK基质),和无组织的WT组织学与三相的组件(第二个面板,基质、芽和上皮结构包括流产肾小球和小管)。10×组成使用Photoshop图像获得和直流(Adobe)整个图像,右面板20 x的图像。C, D) SIX2(红色)和CITED1(绿色)免疫荧光染色和D C) hFK 10编剧的WT # 4。SIX2 + CITED1 + co-expression hFK (C,第二小组)在肾原性的利基(uninduced帽间质,UCM),但没有在发展中(肾脏囊泡,往后推,“s”)和成熟(肾小球及小管)结构。SIX2 + CITED1 +表达式是分散在整个WT (D,第二面板)在芽而不是基质或流产的结构(肾小球及小管)。核与DAPI染色(蓝色),10×组成使用Photoshop图像获得和直流(Adobe)整个图像,右面板20×图像。E) SmartFlare技术验证通过流式细胞术。 SIX2-Cy5 and CITED1-Cy3 probes (top left and right panel respectively) were individually used to isolate cells from hFK (17.4 WGA). Flow cytometry confirmed that 99.7% of SIX2+ cells and 94.3% of CITED1+ cells co-express both mRNA and protein (bottom left and right panels). F) FACS sorting (by Smartflares): 5.96% of cells from hFK 16.4 WGA are SIX2+CITED1+ cells, 0.46% from WT#3 (unfavorable stage I), 8.56% from WT#4 (favorable stage III) and 28.2% from WT#5 (favorable chemotherapy-treated stage IV). G,H) Bulk RNA-seq analysis of hFK (17, 17.2, and 17.5 WGA) and WT (n = 3, as in F). PCA (principal component analysis, G) describes 49.43% and 18.14% of the variability, along PC1 and PC2 respectively, within the expression data set. SIX2+CITED1+ cells from WT cluster independently of SIX2+CITED1+ cells from hFK. H) Hierarchical clustering of total gene expression in SIX2+CITED1+ cells from hFK and WT highlights higher similarity among SIX2+CITED1+ cells from different hFK versus higher divergence of SIX2+CITED1+ cells from different WT. Credit:先进的科学(2023)。DOI: 10.1002 / advs.202206787
“小儿肿瘤可以被认为是发展的癌症,”perinatal博士说,他也是南加州大学凯克医学院的副教授。“正常成人肾缺乏肾前体细胞,因为它们是“筋疲力尽”出生之前。肿瘤,但在霍奇金病引起肾脏功能,这些前体细胞保存,形成肿瘤的质量。”The researchers characterized these Wilms tumor kidney precursor cells, finding that these cells can reproduce the original tumor.
